p53 is commonly referred to as the “guardian of the genome”. Data from multiple experimental models demonstrate a critical and direct role for the p53 family of proteins in protecting organisms from the effects of genetic mutations.

When cells or organisms experience genotoxic or other forms of damage, p53 is activated and binds to a conserved DNA sequence and activates transcription of cell and organism-protective genes. We use genomic and systems concepts to uncover how p53 activates its target genes in reponse to various cellular insults and how cell type, chromatin state, and other factors alter this behavior.

Publications

Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus McCann AA and Sammons MA. bioRxiv 2024

p53motifDB - integration of genomic information and tumor suppressor p53 binding motifs Baniulyte G, Hicks SM, and Sammons MA. bioRxiv 2024

Determinants of p53 DNA binding, gene regulation, and cell fate decisions Fischer M and Sammons MA. Cell Death & Differentiation 2024

p63 and p53 - collaborative partners or dueling rivals? Woodstock, et al. Front. Cell Dev. Biol 2021

Tumor suppressor p53 - from engaging DNA to target gene regulation Sammons MA, et al. Nucleic Acids Research 2020

Control of p53-dependent transcription and enhancer activity by the p53 family member p63 Karsli Uzunbas G, et al. Journal of Biological Chemistry 2019

Comparison of genotoxic vs. non-genotoxic stabilization of p53 provides insight into parallel stress-responsive transcriptional networks Catizone AN, et al. Cell Cycle 2019

Lysine methylation represses p53 activity in teratocarcinoma cancer cells. Zhu J et al. PNAS 2016

A rare DNA contact mutation in cancer confers p53 gain-of-function and tumor cell survival via TNFAIP8 induction. Monteith JA et al. Molecular Oncology 2016

A chromatin-focused siRNA screen for regulators of p53-dependent transcription Sammons MA et al. G3 2016

Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth Zhu J et al. Nature 2015

TP53 engagement with the genome occurs in distinct local chromatin environments via pioneer factor activity Sammons et al. Genome Res 2015